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8 thoughts on “ Sequences - Transient Form (Cassette)

  1. Aug 19,  · Following linearization, the BAC was recircularized by transient induction of gam, bet, and exo to enable recombination between duplicated sequences flanking the kanamycin cassette, resulting in kanamycin loss from the BAC (SI Appendix, Fig. S1, Left). Kanamycin-sensitive clones were then identified via replica plating.
  2. Gene Cassette. Gene cassettes consist of a coding sequence, usually without promoter sequences, followed by an integrase-specific recombination site, and either can exist in a nonfunctional circularized form or are expressed as part of an integron or transposon. From: Principles and Practice of Pediatric Infectious Diseases (Fifth Edition),
  3. Dec 23,  · The efficient expression of therapeutic genes in target cells or tissues is an important component of efficient and safe gene therapy. Utilizing regulatory elements from the human cytokeratin 18 (K18) gene, including 5′ genomic sequences and one of its introns, we have developed a novel expression cassette that can efficiently express reporter genes, as well as the human cystic fibrosis Cited by:
  4. a target sequence when using a transient assay that combines co- cassette under the control of the CaMV 35S promoter. To. tomato ACTIN promoter sequence to form plasmid pGrDL_SPb.
  5. VS, a cassette with an additional bp vector sequence downstream of Tnos terminator Transient EYFP expression patterns of six minimal gene cassettes in sugarcane leaf segments.
  6. (A) n sequence fragments, each flanked by loxP sites (black triangles) in opposing orientations are concatenated to form a cassette. Upon Cre recombination, the cassette is shuffled and pared down until a single fragment, j, remains in either orientation.
  7. Nov 25,  · Such sequences would be true transient expression enhancers, as they function to enhance plasmid nuclear entry, not mRNA production or .
  8. Sep 18,  · Creation of the Synth expression cassette. A novel expression cassette (named Synth) was designed to allow straightforward replacement and testing of different UTRs (see Fig. 1).This Synth cassette is based on the CPMV-HT expression cassette present in the pEAQ-HT vector: it contains the same 35S promoter and nos terminator sequences [], but with synthetic 5′ and 3′UTRs replacing the .

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